협부지방에서 성체 줄기세포의 분리와 골모 세포로의 분화
Differentiation of adult stem cell derived from buccal fat pad into osteoblast
표성운, 박장우, 이일규, 김창현,
소속 상세정보
표성운 ( Pyo Sung-Woon ) - 가톨릭대학교 의과대학 치과학교실 구강악안면외과
박장우 ( Park Jang-Woo ) - 가톨릭대학교 임상치과학대학원 임프란트학과
이일규 ( Lee Il-Kyu ) - 가톨릭대학교 대학원 통합의학전공
김창현 ( Kim Chang-Hyen ) - 가톨릭대학교 의과대학 치과학교실 구강악안면외과
KMID : 0355620060320060524
Abstract
For the repairing of bone defect, autogenous or allogenic bone grafting remains the standard. However, these methods have numerous disadvantages including limited amount, donor site morbidity and spread of diseases. Tissue engineering technique by culturing stem cells may allow for a smart solution for this problem. Adipose tissue contains mesenchymal stem cells that can be differentiate into bone, cartilage, fat or muscle by exposing them to specific growth conditions. In this study, the authors procured the stem cell from buccal fat pad and differentiate them into osteoblast and are to examine the bone induction capacity. Buccal fat-derived cells (BFDC) were obtained from human buccal fat pad and cultured. BFDC were analyzed for presence of stem cell by immunofluorescent staining against CD-34, CD-105 and STRO-1. After BFDC were differentiated in osteogenic medium for three passages, their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase (ALP) staining, Alizarin red staining and RT-PCR for osteocalcin (OC) gene expression. Immunofluorescent and biochemical assays demonstrated that BFDC might be a distinguished stem cells and mineralization was accompanied by increased activity or expression of ALP and OC. And calcium phosphate deposition was also detected in their extracelluar matrix. The current study supports the presence of stem cells within the buccal fat pad and the potential implications for human bone tissue engineering for maxillofacial reconstruction.
키워드
Tissue engineering;Stem cell;Buccal fat pad;Buccal fat derived cell;Osteoblast
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